Teal fluorescent proteins: characterization of a reversibly photoswitchable variant

Hui-wang Ai; Robert E. Campbell

doi:10.1117/12.761423

29 February 2008 Teal fluorescent proteins: characterization of a reversibly photoswitchable variant

Hui-wang Ai, Robert E. Campbell

Proceedings Volume 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 68680D (2008) https://doi.org/10.1117/12.761423
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Fluorescent proteins (FPs) emerged in the mid 1990s as a powerful tool for life science research. Cyan FPs (CFPs), widely used in multicolor imaging or as a fluorescence resonance energy transfer (FRET) donor to yellow FPs (YFPs), were considerably less optimal than other FPs because of some relatively poor photophysical properties. We recently initiated an effort to create improved or alternate versions of CFPs. To address the limitations of CFPs, an alternative known as monomeric teal FP1 (mTFP1) was engineered from the naturally tetrameric Clavularia CFP, by screening either rationally designed or random libraries of variants. mTFP1 has proven to be a particularly useful new member of the FP 'toolbox' by facilitating multicolor live cell imaging. During the directed evolution process of mTFP1, it was noticed that some earlier variants underwent fast and reversible photoisomerization. Some of the initial characterization of one particular mutant, designated as mTFP0.7, is described in this manuscript.

Citation Download Citation

Hui-wang Ai and Robert E. Campbell "Teal fluorescent proteins: characterization of a reversibly photoswitchable variant", Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680D (29 February 2008); https://doi.org/10.1117/12.761423

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