Portable hyperspectral fluorescence imaging system for detection of biofilms on stainless steel surfaces

Won Jun; Kangjin Lee; Patricia Millner; Manan Sharma; Kuanglin Chao; Moon S. Kim

doi:10.1117/12.786870

18 April 2008 Portable hyperspectral fluorescence imaging system for detection of biofilms on stainless steel surfaces

Won Jun, Kangjin Lee, Patricia Millner, Manan Sharma, Kuanglin Chao, Moon S. Kim

Proceedings Volume 6983, Defense and Security 2008: Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display; 698306 (2008) https://doi.org/10.1117/12.786870
Event: SPIE Defense and Security Symposium, 2008, Orlando, Florida, United States

Abstract

A rapid nondestructive technology is needed to detect bacterial contamination on the surfaces of food processing equipment to reduce public health risks. A portable hyperspectral fluorescence imaging system was used to evaluate potential detection of microbial biofilm on stainless steel typically used in the manufacture of food processing equipment. Stainless steel coupons were immersed in bacterium cultures, such as E. coli, Pseudomonas pertucinogena, Erwinia chrysanthemi, and Listeria innocula. Following a 1-week exposure, biofilm formations were assessed using fluorescence imaging. In addition, the effects on biofilm formation from both tryptic soy broth (TSB) and M9 medium with casamino acids (M9C) were examined. TSB grown cells enhance biofilm production compared with M9C-grown cells. Hyperspectral fluorescence images of the biofilm samples, in response to ultraviolet-A (320 to 400 nm) excitation, were acquired from approximately 416 to 700 nm. Visual evaluation of individual images at emission peak wavelengths in the blue revealed the most contrast between biofilms and stainless steel coupons. Two-band ratios compared with the single-band images increased the contrast between the biofilm forming area and stainless steel coupon surfaces. The 444/588 nm ratio images exhibited the greatest contrast between the biofilm formations and stainless coupon surfaces.

Citation Download Citation

Won Jun, Kangjin Lee, Patricia Millner, Manan Sharma, Kuanglin Chao, and Moon S. Kim "Portable hyperspectral fluorescence imaging system for detection of biofilms on stainless steel surfaces", Proc. SPIE 6983, Defense and Security 2008: Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display, 698306 (18 April 2008); https://doi.org/10.1117/12.786870

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available