Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy

Benjamin Rappaz; Elena Cano; Jonas Kühn; Tristan Colomb; Christian Depeursinge; Pierre J. Magistretti; Pierre Marquet

doi:10.1117/12.781565

2 May 2008 Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy

Benjamin Rappaz, Elena Cano, Jonas Kühn, Tristan Colomb, Christian Depeursinge, Pierre J. Magistretti, Pierre Marquet

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 699111 (2008) https://doi.org/10.1117/12.781565
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

Digital holography microscopy (DHM) is an optical microscopy technique which allows recording non-invasively the phase shift induced by living cells with nanometric sensitivity. Here, we exploit the phase signal as an indicator of dry mass (related to the protein concentration). This parameter allows monitoring the protein production rate and its evolution during the cell cycle.

Citation Download Citation

Benjamin Rappaz, Elena Cano, Jonas Kühn, Tristan Colomb, Christian Depeursinge, Pierre J. Magistretti, and Pierre Marquet "Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699111 (2 May 2008); https://doi.org/10.1117/12.781565

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