Implantable optical biosensor for in vivo molecular imaging

Thomas D. O'Sullivan; Elizabeth Munro; Adam de la Zerda; Natesh Parashurama M.D.; Robert Teed; Zachary Walls; Ofer Levi; Sanjiv S. Gambhir; James S. Harris Jr.

doi:10.1117/12.811227

20 February 2009 Implantable optical biosensor for in vivo molecular imaging

Thomas D. O'Sullivan, Elizabeth Munro, Adam de la Zerda, Natesh Parashurama M.D., Robert Teed, Zachary Walls, Ofer Levi, Sanjiv S. Gambhir, James S. Harris Jr.

Author Affiliations +

Proceedings Volume 7173, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX; 717309 (2009) https://doi.org/10.1117/12.811227
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We present the design and fabrication of an implantable fluorescence biosensor suitable for continuously monitored, freely-moving in vivo rodent studies. The GaAs-based semiconductor sensor incorporates an un-cooled photodetector with a 670nm vertical-cavity surface-emitting laser (VCSEL) optimized for sensing fluorescent Cy5.5 dye. For filtering unwanted spectra, a combination of physical and spectral blocking layers yields OD5 excitation rejection at the detector. The sensor detects near-IR fluorescent Cy5.5 molecules in vitro at 100nM concentration (in a 100μL volume) with linear response for concentrations up to 25μM. In a preliminary study in a living mouse, subcutaneously injected dye (1μM Cy5.5 in 50μL) was detected. This technology has the potential to enable new studies of living systems in applications that require long-term, continuous fluorescence sensing.

Citation Download Citation

Thomas D. O'Sullivan, Elizabeth Munro, Adam de la Zerda, Natesh Parashurama M.D., Robert Teed, Zachary Walls, Ofer Levi, Sanjiv S. Gambhir, and James S. Harris Jr. "Implantable optical biosensor for in vivo molecular imaging", Proc. SPIE 7173, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX, 717309 (20 February 2009); https://doi.org/10.1117/12.811227

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