Spectral polarimetry for assessing cell alignment in cultured tissues

James C. Gladish; Donald D. Duncan

doi:10.1117/12.809373

23 February 2009 Spectral polarimetry for assessing cell alignment in cultured tissues

James C. Gladish, Donald D. Duncan

Proceedings Volume 7179, Optics in Tissue Engineering and Regenerative Medicine III; 717909 (2009) https://doi.org/10.1117/12.809373
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Successful development of cultured tissues is heavily influenced by cell alignment within the tissue scaffold. Proper cell alignment leads to optimum tissue strength. It has been demonstrated that proper alignment is engendered by application of physiologically realistic stresses during the cell proliferation process. In situ monitoring of cell alignment during development thus can provide important feedback information in determining the optimum stresses. T-matrix calculations suggest that cell alignment characteristics (cell aspect and orientation) can be inferred from the spectral polarization of light scattered by the cells. Therefore, a spectral polarimetry system has been created to measure these effects to provide feedback for proper cell alignment. In order to properly use the system, a calibration procedure was first established. The calibration procedure entailed making mathematical predictions for the system performance based on the system components, and then empirically validating these predictions. Upon system calibration, measurements were made on a biologically relevant sample. We present results of experimental measurements on the sample and discuss structural inferences made from these measurements. In addition, we compare our results with structural information obtained from histological analysis.

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James C. Gladish and Donald D. Duncan "Spectral polarimetry for assessing cell alignment in cultured tissues", Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 717909 (23 February 2009); https://doi.org/10.1117/12.809373

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KEYWORDS

Calibration

Polarization

Tissues

Polarimetry

Jones calculus

Polarizers

Transmittance

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