In vivo transcranial measurement of light scattering in rat brains during hypoxia

Satoko Kawauchi; Shunichi Sato; Yoichi Uozumi; Hiroshi Nawashiro; Miya Ishihara; Makoto Kikuchi

doi:10.1117/12.807967

24 February 2009 In vivo transcranial measurement of light scattering in rat brains during hypoxia

Satoko Kawauchi, Shunichi Sato, Yoichi Uozumi, Hiroshi Nawashiro, Miya Ishihara, Makoto Kikuchi

Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 71870U (2009) https://doi.org/10.1117/12.807967
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Measurement of intrinsic optical signals (IOSs) is attractive for noninvasive, real-time monitoring of tissue viability in brains. We previously performed measurement of IOSs for a rat global ischemic brain model that was made by rapidly removing blood by saline infusion, and observed that after an induction of ischemia, a unique triphasic change in light scattering occurred. This scattering change preceded the reduction of CuA in cytochrome c oxidase which has been shown to correlate with cerebral ATP decrease. In the present study, we examined whether such triphasic scattering change can be observed in the presence of blood in vivo. Transcranial measurement of diffuse reflectance was performed using a broadband tungsten lamp for a rat brain during hypoxia that was induced by N2 inhalation. The reflectance spectral changes in the visible (500-600 nm) and near-infrared (NIR) (650-850 nm) regions were analyzed to monitor changes in hemodynamics and light scattering, respectively. After starting N2 inhalation, reflectance signals in the visible region showed an increase in deoxy-hemoglobin concentration, and about 80 s after full deoxygenation of hemoglobins, reflectance signals in the NIR region showed a similar triphasic change, which was attributable to change in light scattering. Simultaneous measurement of cerebral EEG showed that neuronal activity ceased about 50 s before this triphasic scattering change. These results show that light scattering will become an important indicator of loss of tissue viability in brain; brain tissue can probably be saved if reoxygenation is achieved before starting this scattering change.

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Satoko Kawauchi, Shunichi Sato, Yoichi Uozumi, Hiroshi Nawashiro, Miya Ishihara, and Makoto Kikuchi "In vivo transcranial measurement of light scattering in rat brains during hypoxia", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 71870U (24 February 2009); https://doi.org/10.1117/12.807967

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KEYWORDS

Light scattering

Reflectivity

Brain

Diffuse reflectance spectroscopy

Scattering

Tissue optics

Hypoxia

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