Developing new fluorescent proteins with stagger extension process

Jie Yang; Jinling Lu; Haiming Luo; Qingming Luo; Zhihong Zhang

doi:10.1117/12.811377

16 February 2009 Developing new fluorescent proteins with stagger extension process

Jie Yang, Jinling Lu, Haiming Luo, Qingming Luo, Zhihong Zhang

Proceedings Volume 7191, Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV; 71910H (2009) https://doi.org/10.1117/12.811377
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The Stagger Extension Process (StEP), a recombination of DNA technique, has been used as a rapid molecular mutagenesis strategy. In this study, for obtaining the fluorescence proteins with new properties, six fluorescence proteins (EYFP, EGFP, ECFP, mCitrine, mCerulean and Venus) were used as the templates to recombine the mutation library by the Stagger Extension Process (StEP) technique. Through screening this mutation library, we have obtained some useful new FPs which are different fluorescent properties with ancestor. These protein will extend fluorescent proteins application.

Citation Download Citation

Jie Yang, Jinling Lu, Haiming Luo, Qingming Luo, and Zhihong Zhang "Developing new fluorescent proteins with stagger extension process", Proc. SPIE 7191, Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV, 71910H (16 February 2009); https://doi.org/10.1117/12.811377

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