The analysis of surface treatment of PDMS on prostate cancer and smooth muscle cells

Cory Huggins; Smitha M. N. Rao; Kytai Nguyen; J.-C. Chiao

doi:10.1117/12.810763

30 December 2008 The analysis of surface treatment of PDMS on prostate cancer and smooth muscle cells

Cory Huggins, Smitha M. N. Rao, Kytai Nguyen, J.-C. Chiao

Proceedings Volume 7269, Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV; 726914 (2008) https://doi.org/10.1117/12.810763
Event: SPIE Smart Materials, Nano- and Micro-Smart Systems, 2008, Melbourne, Australia

Abstract

The analysis of cellular activity when exposed to polydimethylsiloxane (PDMS) is necessary as this material has been used in various applications such as tissue engineering and microfluidic devices for cellular studies due to the polymer's unique mechanical properties. In this particular study, we investigated the effects of corona surface treated PDMS with different cross-linker ratios on cellular activities by analyzing prostate cancer cell (PC-3) and vascular smooth muscle cell (VSMC) adhesion and proliferation. Both cell lines were subjected to a thin PDMS layer immediately after and 24 hours after corona treatment. The results indicated steady cell adhesion and proliferation rates for both smooth muscle and prostate cancer cells when seeded onto PDMS 24 hours after corona surface treatment, but significantly less cell adhesion when seeded immediately after activation and controls (PDMS without any treatment). These results would allow future PC-3 and VSMC experiments to be performed in a PDMS environment that is not detrimental for adhesion and proliferation.

Citation Download Citation

Cory Huggins, Smitha M. N. Rao, Kytai Nguyen, and J.-C. Chiao "The analysis of surface treatment of PDMS on prostate cancer and smooth muscle cells", Proc. SPIE 7269, Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV, 726914 (30 December 2008); https://doi.org/10.1117/12.810763

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available