Bacterial mixture analysis with Raman chemical imaging microspectroscopy

Ashish Tripathi; Rabih E. Jabbour; Jason A. Guicheteau; Steven D. Christesen; Darren K. Emge; Janet L. Jensen; A. Peter Snyder

doi:10.1117/12.806456

8 May 2009 Bacterial mixture analysis with Raman chemical imaging microspectroscopy

Ashish Tripathi, Rabih E. Jabbour, Jason A. Guicheteau, Steven D. Christesen, Darren K. Emge, Janet L. Jensen, A. Peter Snyder

Author Affiliations +

Proceedings Volume 7304, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X; 730403 (2009) https://doi.org/10.1117/12.806456
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

Raman chemical imaging microspectroscopy (RCIM) is being evaluated as a technology for waterborne pathogen detection. Binary and ternary mixtures including combinations of polystyrene beads, Grampositive Bacillus anthracis and B. atrophaeus spores, B. cereus vegetative cells, and Gram-negative E. coli cells were investigated by RCIM for differentiation and characterization purposes. We have demonstrated the ability of RCIM, in combination with Pearson's cross correlation and multivariate principal components analysis data reduction techniques, to differentiate these components in the same field of view (FOV). Conventional applications of RCIM consist of differentiating relatively broad areas in a FOV. Here, RCIM is expanded in its capabilities to differentiate and distinguish between different micron size species in single particles and clusters of mixed species.

Citation Download Citation

Ashish Tripathi, Rabih E. Jabbour, Jason A. Guicheteau, Steven D. Christesen, Darren K. Emge, Janet L. Jensen, and A. Peter Snyder "Bacterial mixture analysis with Raman chemical imaging microspectroscopy", Proc. SPIE 7304, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X, 730403 (8 May 2009); https://doi.org/10.1117/12.806456

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KEYWORDS

Raman spectroscopy

Picosecond phenomena

Particles

Imaging spectroscopy

Bacteria

Microscopes

Chemical analysis

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