Optofluidic ring resonator sensor for sensitive label-free detection of breast cancer antigen CA15-3 in human serum

Hongying Zhu; Paul S. Dale; Xudong Fan

doi:10.1117/12.818257

29 April 2009 Optofluidic ring resonator sensor for sensitive label-free detection of breast cancer antigen CA15-3 in human serum

Hongying Zhu, Paul S. Dale, Xudong Fan

Author Affiliations +

Proceedings Volume 7322, Photonic Microdevices/Microstructures for Sensing; 732204 (2009) https://doi.org/10.1117/12.818257
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

Breast cancer is the most frequently diagnosed malignancy in women worldwide. Because of its great impact on society, a lot of research funding has been used to develop novel detection tools for aiding breast cancer diagnosis and prognosis. In this work, we demonstrated a simple, fast, and sensitive detection of circulating breast cancer biomarker CA15-3 with opto-fluidic ring resonator (OFRR) sensors. The OFRR sensor employs a thin-walled capillary with wall thickness less than 4 μm. The circular cross section of the capillary forms the optical ring resonator, in which the light circulates in the form of whispering gallery modes (WGMs). The capillary wall is thin enough that the evanescent field of the WGM extends into the capillary core and responds to refractive index changes in the capillary core or close to its interior surface. The WGM spectral position will change when the biomolecules bind to the surface, yielding quantitative and kinetic information about the biomolecule interaction. Here, the direct immunoassay method was employed for the detection of CA15-3 antigen without any signal amplification steps. The sensor performance in both PBS buffer and human serum were investigated, respectively. The experimental detection limit was 5 units/mL in PBS buffer and 30 units/mL for CA15-3 spiked in serum, both of which satisfied clinical diagnosis requirements. The potential use of the OFRR as the point-of-care device for breast cancer detection was tested by measuring the CA15-3 level in blood samples collected from stage IV breast cancer patients and the results were compared with standard clinical test.

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Hongying Zhu, Paul S. Dale, and Xudong Fan "Optofluidic ring resonator sensor for sensitive label-free detection of breast cancer antigen CA15-3 in human serum", Proc. SPIE 7322, Photonic Microdevices/Microstructures for Sensing, 732204 (29 April 2009); https://doi.org/10.1117/12.818257

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