In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region

Slávka Kascáková; Sebastiaan de Visscher; Bastiaan Kruijt; Henriëtte S. de Bruijn; Angélique van der Ploeg-van den Heuvel; Henricus J. C. M. Sterenborg; Max J. H. Witjes; Arjen Amelink; Dominic J. Robinson

doi:10.1117/12.822947

14 July 2009 In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region

Slávka Kascáková, Sebastiaan de Visscher, Bastiaan Kruijt, Henriëtte S. de Bruijn, Angélique van der Ploeg-van den Heuvel, Henricus J. C. M. Sterenborg, Max J. H. Witjes, Arjen Amelink, Dominic J. Robinson

Author Affiliations +

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 738063 (2009) https://doi.org/10.1117/12.822947
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

In this study, a ratiometric quantification method is developed and applied to monitor mesotetra(hydroxyphenyl) chlorin (mTHPC) pharmacokinetics in the rat skin-fold observation chamber. The method employs a combination of dual-wavelength excitation and dual-wavelength detection. The excitation and detection wavelengths were selected in close to NIR. The first excitation wavelength was used to excite the mTHPC and autofluorescence and the second to excite only autofluorescence, so that this could be substracted. Subsequently the difference was divided by the autofluorescence. Since the method applies division of signal with no mTHPC fluorescence, theory suggests on linear dependency of the method on photosensitizer concentration.

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Slávka Kascáková, Sebastiaan de Visscher, Bastiaan Kruijt, Henriëtte S. de Bruijn, Angélique van der Ploeg-van den Heuvel, Henricus J. C. M. Sterenborg, Max J. H. Witjes, Arjen Amelink, and Dominic J. Robinson "In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738063 (14 July 2009); https://doi.org/10.1117/12.822947

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KEYWORDS

Luminescence

Tissues

Tissue optics

Absorption

Near infrared

Fluorescence spectroscopy

In vivo imaging

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