Development of an optomicrofluidic flow cytometer for the sorting of stem cells from blood samples

Md. Z. Islam; Xuantao Su; Sean E. Kirkwood; Kirat Singh; James N. McMullin; Wojciech Rozmus; Anna Janowska-Wieczorek; Ying Y. Tsui

doi:10.1117/12.839802

4 August 2009 Development of an optomicrofluidic flow cytometer for the sorting of stem cells from blood samples

Md. Z. Islam, Xuantao Su, Sean E. Kirkwood, Kirat Singh, James N. McMullin, Wojciech Rozmus, Anna Janowska-Wieczorek, Ying Y. Tsui

Author Affiliations +

Proceedings Volume 7386, Photonics North 2009; 73860C (2009) https://doi.org/10.1117/12.839802
Event: Photonics North 2009, 2009, Quebec, Canada

Abstract

In this paper, we report the preliminary development of a fiber coupled microfluidic flow cytometer with its potential application of sorting the very small embryonic like (VSEL) stem cells out of a mixture of platelets and VSEL stem cells. The identification of a VSEL stem cell from a platelet is based on the large difference of their abilities to scatter light. A simple cytometer prototype was built by cutting the fluidic and other channels into a polymer sheet and bonding it with epoxy between two standard glass slides. Standard photolithography was used to expose an observation window over the upper coated glass to reduce background scattered light. Liquid sample containing micro-particles (such as cells) is injected into the microfluidic channel. Light from a 532-nm CW diode laser is coupled into the optical fiber that delivers the light to the detection region in the channel to interrogate the flowing-by micro-particles. The scattering light from the interrogated micro-particle is collected by a photodiode placed over the observation window. The device sorts the micro-particle into the sort or waste outlet depending on the level of the photodiode signal. We used fluorescent latex beads to test the detection and sorting functionalities of the device. It was found that the system could only detect about half of the beads but could sort almost all the beads it detected.

Citation Download Citation

Md. Z. Islam, Xuantao Su, Sean E. Kirkwood, Kirat Singh, James N. McMullin, Wojciech Rozmus, Anna Janowska-Wieczorek, and Ying Y. Tsui "Development of an optomicrofluidic flow cytometer for the sorting of stem cells from blood samples", Proc. SPIE 7386, Photonics North 2009, 73860C (4 August 2009); https://doi.org/10.1117/12.839802

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