Hydrogen peroxide generated by NADPH oxidase is involved in high blue-light-induced chloroplast avoidance movements in Arabidopsis

Feng Wen; Da Xing; Lingrui Zhang

doi:10.1117/12.841575

28 October 2009 Hydrogen peroxide generated by NADPH oxidase is involved in high blue-light-induced chloroplast avoidance movements in Arabidopsis

Feng Wen, Da Xing, Lingrui Zhang

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Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75190M (2009) https://doi.org/10.1117/12.841575
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

One of the most important functions of blue light is to induce chloroplast movements by reducing the damage to photosynthetic machinery under excess light. Hydrogen peroxide (H₂O₂), generated by various environmental stimuli, can act as a signaling molecule that regulates a number of developmental processes and environmental responses. To investigate whether H₂O₂ is involved in high blue light-induced chloroplast avoidance movements, we use luminescence spectrometer to observe H₂O₂ generation with the assistance of the fluorescence probe dichlorofluorescin diacetate (H2DCF-DA). After treatment with high blue light, a large quantity of H₂O₂ indicated by the fluorescence intensity of DCF is produced in a dose-dependent manner in leaf strip of Arabidopsis. Enzymatic assay shows that the activity of NADPH oxidase, which is a major site for H₂O₂ generation, also rapidly increases in treated strips. Exogenously applied H₂O₂ can promote the high blue light-induced chloroplast movements. Moreover, high blue light-induced H₂O₂ generation can be abolished completely by addition of exogenous catalase (CAT), and partly by diphenylene iodonium (DPI) and dichlorophenyl dimethylurea (DCMU), which are an NADPH oxidase inhibitor and a blocker of electron transport chain. And subsequent chloroplast movements can be abolished by CAT and DPI, but not by DCMU. These results presented here suggested that high blue light can induce oxidative burst, and NADPH oxidase as a major producer for H₂O₂ is involved in blue light-induced chloroplast avoidance movements.

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Feng Wen, Da Xing, and Lingrui Zhang "Hydrogen peroxide generated by NADPH oxidase is involved in high blue-light-induced chloroplast avoidance movements in Arabidopsis", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190M (28 October 2009); https://doi.org/10.1117/12.841575

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KEYWORDS

Transmittance

Luminescence

Computed tomography

Spectroscopy

Hydrogen

Plasma

Oxygen

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