Visualization of reproduction toxicity of QDs for in vitro oocytes maturation

Gaixia Xu; Xiaotan Lin; Ken-Tye Yong; Indrajit Roy; Junle Qu; Xiaomei Wang

doi:10.1117/12.843521

28 October 2009 Visualization of reproduction toxicity of QDs for in vitro oocytes maturation

Gaixia Xu, Xiaotan Lin, Ken-Tye Yong, Indrajit Roy, Junle Qu, Xiaomei Wang

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75191W (2009) https://doi.org/10.1117/12.843521
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

Recently, QDs have attracted enormous attention for their potential applications ranging from physics to medicine. However, the toxicity of QDs impends its development in clinics experiment. In this work, we investigated the reproduction toxicity of QDs for in vitro oocytes matureation. The immatured oocytes of 28 day Kunming mice were harvested and cultured in vitro. And the biocompatible lysine-coated CdSe/CdS/ZnS QDs were incubated with oocytes for a certain periods. Then, each single oocyte-cumulus-complex was visualized under Leica scanning confocal microscope and rendered the 3-dimensional image. After 24 hrs, the maturation rate of oocytes decreased obviously (from 62% to 21.8%) with the concentration of QDs increasing. The 3D rendered images of oocyte-cumulus-complex showed that the most QDs distributed inside the cumulus, but no QDs entered oocytes. In summary, the results suggeste that the oocytes maturation process has high susceptibility for disturbances of QDs. The more QDs were uptaken by cumulus cells, the lower maturation rate of in vitro oocytes. We presume the QD interfere the process of oocytes maturation by dysfunctioning the cumulus cells or disturb the signal-interaction between germ cell and somatic cell. To our best knowledge, this is the first time that 3D visulization methods are used to analysize the reproduction toxicity of in vitro oocytes. The further toxicity mechanism of QDs for oocytes in vitro is undergoing investigation.

Citation Download Citation

Gaixia Xu, Xiaotan Lin, Ken-Tye Yong, Indrajit Roy, Junle Qu, and Xiaomei Wang "Visualization of reproduction toxicity of QDs for in vitro oocytes maturation", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191W (28 October 2009); https://doi.org/10.1117/12.843521

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available