Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin; Shiro Urayama M.D.; Ramez M. G. Saroufeem M.D.; Dennis L. Matthews; Stavros G. Demos

doi:10.1117/12.840715

11 February 2010 Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin, Shiro Urayama M.D., Ramez M. G. Saroufeem M.D., Dennis L. Matthews, Stavros G. Demos

Proceedings Volume 7561, Optical Biopsy VII; 756103 (2010) https://doi.org/10.1117/12.840715
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

Citation Download Citation

Bevin Lin, Shiro Urayama M.D., Ramez M. G. Saroufeem M.D., Dennis L. Matthews, and Stavros G. Demos "Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation", Proc. SPIE 7561, Optical Biopsy VII, 756103 (11 February 2010); https://doi.org/10.1117/12.840715

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