The effect of temperature of fluorescence: an animal study

Alex Walsh; Bart Masters; Duco Jansen; A. J. Welch; Anita Mahadevan-Jansen

doi:10.1117/12.842578

11 February 2010 The effect of temperature of fluorescence: an animal study

Alex Walsh, Bart Masters, Duco Jansen, A. J. Welch, Anita Mahadevan-Jansen

Proceedings Volume 7562, Optical Interactions with Tissues and Cells XXI; 75620W (2010) https://doi.org/10.1117/12.842578
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The effect of temperature on the fluorescence of enucleated porcine eyes and rat skin was studied. The fluorescence peak intensity was found to decrease as the tissue temperature increased. A dual-excitation, fiber-based system was used to collect fluorescence and diffuse-reflectance spectra from the samples. A thermal camera was used to determine the temperature of the tissue at the time of fluorescence measurement. The samples were mounted in a saline bath and measurements were made as the tissue temperature was increased from -20°C to 70°C. Results indicate that temperature affects several fluorescence spectra characteristics. The peak height decreased as temperature increased. At temperatures above 60°C, the peak position shifted to lower wavelengths. Heating and cooling experiments of the rat skin demonstrate the recovery of the loss in fluorescence. The diffuse reflectance spectra indicated a change in optical properties past 60°C, but prior to the denaturation temperature for collagen at 57°C, no change in optical properties was observed. Results suggest that the decrease in fluorescence is both a property of fluorescence and a result of altering optical properties.

Citation Download Citation

Alex Walsh, Bart Masters, Duco Jansen, A. J. Welch, and Anita Mahadevan-Jansen "The effect of temperature of fluorescence: an animal study", Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 75620W (11 February 2010); https://doi.org/10.1117/12.842578

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KEYWORDS

Luminescence

Temperature metrology

Tissues

Optical properties

Diffuse reflectance spectroscopy

Skin

Eye

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