Analysis of GFP-FOXO3a nuclear-cytoplasmic shuttling in ASTC-a-1 cells under growth factor stimulation

Xianwang Wang; Da Xing; Wei R. Chen

doi:10.1117/12.841140

11 February 2010 Analysis of GFP-FOXO3a nuclear-cytoplasmic shuttling in ASTC-a-1 cells under growth factor stimulation

Xianwang Wang, Da Xing, Wei R. Chen

Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650O (2010) https://doi.org/10.1117/12.841140
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

FOXO transcription factors are important regulators of cell survival in response to a variety of stimuli, among which are hypoxic stress, oxidative stress, and growth factor deprivation. Subcellular localization of FOXO proteins plays a major role in the regulation of their activity. In this study, using confocal imaging of the cells transfected with GFP-FOXO3a and fluorescence recovery after photobleaching technique, we visualized the dynamic nuclear translocation of GFP-FOXO3a in ASTC-a-1 cells under growth factor stimulus. In healthy cells, GFP-FOXO3a was well-distributed in the cytoplasm or widespread distributed in the cytoplasm and the nucleus but the cytoplasm was significantly more than the nucleus. Deprivation of growth factor, we monitored the nuclear localization of GFP-FOXO3a and the dynamic translocation of it from cytoplasm to nucleus. Interestingly, upon stimulation with growth factor in cells again, we visualized the dynamic nuclear exclusion of GFP-FOXO3a and cytoplasm distribution rapidly. In conclusion, these results demonstrated that FOXO3a can reversible shuttling between cytoplasm and nucleus upon stimulation with growth factor.

Citation Download Citation

Xianwang Wang, Da Xing, and Wei R. Chen "Analysis of GFP-FOXO3a nuclear-cytoplasmic shuttling in ASTC-a-1 cells under growth factor stimulation", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650O (11 February 2010); https://doi.org/10.1117/12.841140

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