Evaluation of human serum of severe rheumatoid arthritis by confocal Raman spectroscopy

C. S. Carvalho; L. Raniero; A. M. E. Santo; M. M. Pinheiro; L. E. C. Andrade; M. A. G. Cardoso; J. S. Junior; A. A. Martin

doi:10.1117/12.840808

26 February 2010 Evaluation of human serum of severe rheumatoid arthritis by confocal Raman spectroscopy

C. S. Carvalho, L. Raniero, A. M. E. Santo, M. M. Pinheiro, L. E. C. Andrade, M. A. G. Cardoso, J. S. Junior, A. A. Martin

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Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 756823 (2010) https://doi.org/10.1117/12.840808
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Rheumatoid Arthritis is a systemic chronic inflammatory disease, recurrent and systemic, initiated by autoantibodies and maintained by inflammatory mechanisms cellular applicants. The evaluation of this disease to promote early diagnosis, need an associations of many tools, such as clinical, physical examination and thorough medical history. However, there is no satisfactory consensus due to its complexity. In the present work, confocal Raman spectroscopy was used to evaluate the biochemical composition of human serum of 40 volunteers, 24 patients with rheumatoid arthritis presenting clinical signs and symptoms, and 16 healthy donors. The technique of latex agglutination for the polystyrene covered with human immunoglobulin G and PCR (protein c-reactive) was performed for confirmation of possible false-negative results within the groups, facilitating the statistical interpretation and validation of the technique. This study aimed to verify the changes for the characteristics Raman peaks of biomolecules such as immunoglobulins amides and protein. The results were highly significant with a good separation between groups mentioned. The discriminant analysis was performed through the principal components and correctly identified 92% of the donors. Based on these results, we observed the behavior of arthritis autoimmune, evident in certain spectral regions that characterize the serological differences between the groups.

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C. S. Carvalho, L. Raniero, A. M. E. Santo, M. M. Pinheiro, L. E. C. Andrade, M. A. G. Cardoso, J. S. Junior, and A. A. Martin "Evaluation of human serum of severe rheumatoid arthritis by confocal Raman spectroscopy", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756823 (26 February 2010); https://doi.org/10.1117/12.840808

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KEYWORDS

Raman spectroscopy

Confocal microscopy

Proteins

Diagnostics

Statistical analysis

Blood

Principal component analysis

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