Optical forces in biophotonics: transfection and cell sorting

Wolfgang A. Ertmer; Heiko Meyer; Raoul Lorbeer; Gerald Bergmann; Christina Krämer; Holger Lubatschowski; Hugo Murua Escoboar; Ingo Nolte; Alexander Heisterkamp

doi:10.1117/12.841868

8 February 2010 Optical forces in biophotonics: transfection and cell sorting

Wolfgang A. Ertmer, Heiko Meyer, Raoul Lorbeer, Gerald Bergmann, Christina Krämer, Holger Lubatschowski, Hugo Murua Escoboar, Ingo Nolte, Alexander Heisterkamp

Author Affiliations +

Proceedings Volume 7613, Complex Light and Optical Forces IV; 76130Q (2010) https://doi.org/10.1117/12.841868
Event: SPIE OPTO, 2010, San Francisco, California, United States

Abstract

Lately, several groups successfully used ultrashort laser pulses to selectively permeabilize the membrane of living cells to achieve transport of foreign molecules, like DNA, into the cells. For this, the high field intensities of tightly focused laser pulses are used to induce multiphoton absorption and the creation of a small scale optical breakdown at the membrane of the target cell. Afterwards, DNA or other foreign molecules are able to diffuse into the cell and achieve, for example, transfection of living cells. However, the cell throughput of this method is low, as, due to tight focusing. We present a technique to achieve fs-laser transfection in living cells at higher throughput by implementing optical traps into microfluidic chips. For this, a trapping laser beam, is coupled into a microscope setup and combined with a Ti:Sa fslaser beam to achieve simultaneous trapping and optical perforation.

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Wolfgang A. Ertmer, Heiko Meyer, Raoul Lorbeer, Gerald Bergmann, Christina Krämer, Holger Lubatschowski, Hugo Murua Escoboar, Ingo Nolte, and Alexander Heisterkamp "Optical forces in biophotonics: transfection and cell sorting", Proc. SPIE 7613, Complex Light and Optical Forces IV, 76130Q (8 February 2010); https://doi.org/10.1117/12.841868

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