Quantification of fluorescent spots in time series of 3D confocal microscopy images of endoplasmic reticulum exit sites based on the HMAX transform

Petr Matula; Fatima Verissimo; Stefan Wörz; Roland Eils; Rainer Pepperkok; Karl Rohr

doi:10.1117/12.844441

9 March 2010 Quantification of fluorescent spots in time series of 3D confocal microscopy images of endoplasmic reticulum exit sites based on the HMAX transform

Petr Matula, Fatima Verissimo, Stefan Wörz, Roland Eils, Rainer Pepperkok, Karl Rohr

Author Affiliations +

Proceedings Volume 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging; 76261H (2010) https://doi.org/10.1117/12.844441
Event: SPIE Medical Imaging, 2010, San Diego, California, United States

Abstract

We present an approach for the quantification of fluorescent spots in time series of 3-D confocal microscopy images of endoplasmic reticulum exit sites of dividing cells. Fluorescent spots are detected based on extracted image regions of highest response using the HMAX transform and prior convolution of the 3-D images with a Gaussian kernel. The sensitivity of the involved parameters was studied and a quantitative evaluation using both 3-D synthetic and 3-D real data was performed. The approach was successfully applied to more than one thousand 3-D confocal microscopy images.

Citation Download Citation

Petr Matula, Fatima Verissimo, Stefan Wörz, Roland Eils, Rainer Pepperkok, and Karl Rohr "Quantification of fluorescent spots in time series of 3D confocal microscopy images of endoplasmic reticulum exit sites based on the HMAX transform", Proc. SPIE 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging, 76261H (9 March 2010); https://doi.org/10.1117/12.844441

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