Protoporphyrin IX for photodynamic therapy of brain tumours

A. Johansson; F. W. Kreth; A. Ardeshiri; W. Stummer; O. Schnell; J. Herms; G. Palte; W. Beyer; H. Stepp

doi:10.1117/12.854335

17 May 2010 Protoporphyrin IX for photodynamic therapy of brain tumours

A. Johansson, F. W. Kreth, A. Ardeshiri, W. Stummer, O. Schnell, J. Herms, G. Palte, W. Beyer, H. Stepp

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77151M (2010) https://doi.org/10.1117/12.854335
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

Protoporphyrin IX (PpIX) displays high tumour-selective uptake following oral administration of 5-aminolaevulinic acid (ALA), a fact that is being exploited for the fluorescence-guided resection (FGR) and photodynamic therapy (PDT) of human brain malignancies. A clinical procedure for interstitial PDT (iPDT) has been established including pre-treatment planning, optical fiber insertion under stereotactic guidance and therapeutic irradiation at non-thermal fluence rates. We have previously reported on median survival in the range of 15 months and the existence of some intriguing long-term survivors (>5 years) following iPDT. Such successful treatments rely on for example sufficient light, PpIX and oxygen levels. We have investigated the absolute PpIX concentration as well as the PDT-induced photobleaching kinetics in brain tissues. Tissue samples acquired during FGR contained PpIX concentrations up to 28 μM. This observation implies that ALA-induced PpIX levels are sufficient for inducing PDT effects in viable tumour tissue upon therapeutic irradiation. However, regions of pre-existing necrosis were characterised by significantly lower photosensitiser levels. Fluorescence spectroscopy was implemented in parallel to iPDT with the aim to employ PpIX photobleaching as a tool for realtime treatment supervision and early treatment prognosis.

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A. Johansson, F. W. Kreth, A. Ardeshiri, W. Stummer, O. Schnell, J. Herms, G. Palte, W. Beyer, and H. Stepp "Protoporphyrin IX for photodynamic therapy of brain tumours", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151M (17 May 2010); https://doi.org/10.1117/12.854335

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