Cyanobacteria gene and protein sequences in diurnal oscillation metabolic processes

George Tremberger Jr.; T. Holden; E. Cheung; S. Dehipawala; N. Gadura; U. Golebiewska; K. Valentin; M. Smulczeski; W. Satizabal; P. Schneider; D. Lieberman; T. Cheung

doi:10.1117/12.860093

7 September 2010 Cyanobacteria gene and protein sequences in diurnal oscillation metabolic processes

George Tremberger Jr., T. Holden, E. Cheung, S. Dehipawala, N. Gadura, U. Golebiewska, K. Valentin, M. Smulczeski, W. Satizabal, P. Schneider, D. Lieberman, T. Cheung

Author Affiliations +

Proceedings Volume 7819, Instruments, Methods, and Missions for Astrobiology XIII; 78190U (2010) https://doi.org/10.1117/12.860093
Event: SPIE Optical Engineering + Applications, 2010, San Diego, California, United States

Abstract

Daytime photosynthesis and nighttime nitrogen fixation metabolic processes have been reported in the bacterium, Cyanothece 51142. The organism's auto-fluorescence with 532 nm excitation would place cyanobacteria at the forefront in the remote sensing of microbial activity in astrobiology. The sensitivity of nitrogenase to oxygen was studied in terms of sequence nucleotide fluctuation. A nucleotide sequence fractal dimension can be calculated from a numerical series consisting of the atomic numbers of each nucleotide. The fractal dimension and Shannon entropy form a two-dimensional measure that is useful in assessing evolutionary pressures. The studied sequences include nitrogenase iron protein NifH, nitrogenase molybdenum-iron protein alpha chain NifD and beta chain NifK. The photosynthesis-lacking UCYN-A cyanobacterium as reported recently in the journal, Nature, was observed to have the lowest entropy with relatively high fractal dimension values in the studied NifH, NifD and NifH sequences. The fractal dimension of NifH sequences correlates with the NifD sequence values with an R-square of 0.91 (N = 8). The Shannon mononucleotide entropy of NifD sequences correlates with the NifK sequence values with an R-square value of 0.92 (N = 8). The observed strong correlation suggests the presence of gradual evolutionary pressure among the studied cyanobacteria, and throws light on the reported paradox in evolution for the case of UCYN-A. The results show that diurnal oscillation metabolic processes in cyanobacteria (including the photosynthesis-deficient case) are not associated with extraordinary evolutionary pressures and thus are processes consistent with putative astrobiological organisms.

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George Tremberger Jr., T. Holden, E. Cheung, S. Dehipawala, N. Gadura, U. Golebiewska, K. Valentin, M. Smulczeski, W. Satizabal, P. Schneider, D. Lieberman, and T. Cheung "Cyanobacteria gene and protein sequences in diurnal oscillation metabolic processes", Proc. SPIE 7819, Instruments, Methods, and Missions for Astrobiology XIII, 78190U (7 September 2010); https://doi.org/10.1117/12.860093

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KEYWORDS

Fractal analysis

Proteins

Biology

Iron

Organisms

Photosynthesis

Image information entropy

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