Light induced heme oxygenase 1 is suppressed by Bach1 in human skin keratinocytes

Ruoqi Xu; Wanqian Liu; Jin Zhang; Li Yang; Linhong Deng; Rex M. Tyrrell; Julia Li Zhong

doi:10.1117/12.870984

8 November 2010 Light induced heme oxygenase 1 is suppressed by Bach1 in human skin keratinocytes

Ruoqi Xu, Wanqian Liu, Jin Zhang, Li Yang, Linhong Deng, Rex M. Tyrrell, Julia Li Zhong

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78450Z (2010) https://doi.org/10.1117/12.870984
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Ultraviolet A (UVA) radiation is an oxidizing agent that strongly induces the heme oxygenase 1(HO-1) expression in cultured human skin fibroblasts, but weakly induces it in skin keratinocytes. Here, we report that low basal levels of HO- 1 and much higher basal levels of HO-2 protein were observed in keratinocytes compared with fibroblasts. Silencing of Bach1 strongly increased HO-1 levels in HaCaT transformed keratinocytes and these HO-1 levels were not further increased by either UVA irradiation or silencing of HO-2. This is consistent with the conclusion that high constitutive levels of HO-2 expression in keratinocytes are responsible for the resistance of these cells to HO-1 induction by UVA radiation and that Bach1 plays a predominant role in influencing the lack of HO-1 expression in keratinocytes. Bach1 inhibition reduced the 500 kJ/m² UVA-induced cell damage by LDH membrane integrity and MTS viability assays. These results suggest that Bach1 inhibition protect against high dose of UVA irradiation induced damage in keratinocytes.

Citation Download Citation

Ruoqi Xu, Wanqian Liu, Jin Zhang, Li Yang, Linhong Deng, Rex M. Tyrrell, and Julia Li Zhong "Light induced heme oxygenase 1 is suppressed by Bach1 in human skin keratinocytes", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450Z (8 November 2010); https://doi.org/10.1117/12.870984

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KEYWORDS

Proteins

Skin

Cell death

Antimony

Lithium

Resistance

Ultraviolet radiation

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