Fluorescence lifetime imaging using multi-dimension time-correlated single photon counting method

Cuixia Sheng; Heijing Tang

doi:10.1117/12.868319

8 November 2010 Fluorescence lifetime imaging using multi-dimension time-correlated single photon counting method

Cuixia Sheng, Heijing Tang

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78451V (2010) https://doi.org/10.1117/12.868319
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

The key technology of fluorescence lifetime imaging is introduced in this article. Time-correlated single photon counting (TCSPC) in Time-domain method is adopted to record fluorescence lifetime . The principle of TCSPC and the Multi-wavelength imaging method are introduced in the paper. The counting efficiency and the amount of information in the data can be increased by recording the fluorescence in several wavelength channels simultaneously. Multi-wavelength imaging was also successfully used to separate different chromophores in stead-state images.

Citation Download Citation

Cuixia Sheng and Heijing Tang "Fluorescence lifetime imaging using multi-dimension time-correlated single photon counting method", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451V (8 November 2010); https://doi.org/10.1117/12.868319

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