A pilot characterization of quantitative time-domain fluorescence diffuse optical tomography

Jiao Li; Feng Gao; Xi Yi; Xin Wang; Linhui Wu; Pingping Zhu; Limin Zhang; Huijuan Zhao

doi:10.1117/12.874102

1 March 2011 A pilot characterization of quantitative time-domain fluorescence diffuse optical tomography

Jiao Li, Feng Gao, Xi Yi, Xin Wang, Linhui Wu, Pingping Zhu, Limin Zhang, Huijuan Zhao

Proceedings Volume 7896, Optical Tomography and Spectroscopy of Tissue IX; 78961G (2011) https://doi.org/10.1117/12.874102
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Quantitative measurements of fluorescent parameters have merited great interest lately for near-infrared fluorescence diffuse optical tomography - the efficient small animal imaging tool. We present a two-dimensional image reconstruction method for time-domain fluorescence diffuse optical tomography, which employs the analytical solution to the Laplace-transformed time-domain photon-diffusion equation to construct the inverse model and introduces a pair of real-domain transform-factors to effectively separate the fluorescent yield and lifetime parameters from the algebraic reconstruction technique solutions to the resultant linear inversions. By use of a specifically designed a multi-channel time-correlated single photon counting system and a normalized Born formulation for the inversion, the proposed scheme in a circular domain is experimentally validated using small-animal-sized cylindrical phantoms that embed several fluorescent targets made from 1%-Intralipid solution and differently contrasting fluorescent agents, where the time-resolved excitation and fluorescence signals are measured on the boundary. The results show that the approach retrieves the positions and shapes of the targets with a reasonable accuracy and simultaneously achieve quantitative reconstruction of the fluorescent yield and lifetime.

Citation Download Citation

Jiao Li, Feng Gao, Xi Yi, Xin Wang, Linhui Wu, Pingping Zhu, Limin Zhang, and Huijuan Zhao "A pilot characterization of quantitative time-domain fluorescence diffuse optical tomography", Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 78961G (1 March 2011); https://doi.org/10.1117/12.874102

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