Effects of He-Ne laser irradiation on red blood cells in vitro

Vijay H. Ghadage; Gauri R. Kulkarni

doi:10.1117/12.874029

18 February 2011 Effects of He-Ne laser irradiation on red blood cells in vitro

Vijay H. Ghadage, Gauri R. Kulkarni

Proceedings Volume 7897, Optical Interactions with Tissue and Cells XXII; 78970I (2011) https://doi.org/10.1117/12.874029
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Laser radiation has many applications in biomedical field, such as wound healing, tissue repairing, heating and ablation processes. Intravenous low power laser radiation is used clinically for skin and vascular disorders. Laser radiation improves microcirculation and modulates the rheological properties of blood. FTIR (Fourier Transform Infra Red Spectra) is used to see the structural changes in erythrocyte membrane. In the present work He Ne laser (λ= 632nm, power=2mW) is used to irradiate human Red blood cells. Red blood cells are separated from human whole blood using centrifugation method (time=10 min., temperature=15°C and RPM=3000) and then exposed to HeNe laser radiation. Laser exposure time is varied from 10 min. to 40min for Red blood cells. Absorption spectrum, FTIR and fluorescence spectra of RBC are compared before and after HeNe laser irradiation. The absorption spectrum of RBC after exposure to HeNe laser shows a significant decrease in absorbance. The FTIR spectrum of non irradiated RBC clearly show the peaks due to O-H (free group), C=O (amide I group), N=O (nitro group), C-O (anhydride group) and C-H (aromatic group). Laser radiation changes in transmittance in FTIR spectra related to C=O group and percentage of transmittance increases for O-H, C=C, N=O, C-O and C-H group.

Citation Download Citation

Vijay H. Ghadage and Gauri R. Kulkarni "Effects of He-Ne laser irradiation on red blood cells in vitro", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970I (18 February 2011); https://doi.org/10.1117/12.874029

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