Traumatic brain injury caused by laser-induced shock wave in rats: a novel laboratory model for studying blast-induced traumatic brain injury

Ben Hatano; Yoshihisa Matsumoto; Naoki Otani; Daizoh Saitoh; Shinichi Tokuno; Yasushi Satoh; Hiroshi Nawashiro; Yoshitaro Matsushita; Shunichi Sato

doi:10.1117/12.875909

18 February 2011 Traumatic brain injury caused by laser-induced shock wave in rats: a novel laboratory model for studying blast-induced traumatic brain injury

Ben Hatano, Yoshihisa Matsumoto, Naoki Otani, Daizoh Saitoh, Shinichi Tokuno, Yasushi Satoh, Hiroshi Nawashiro, Yoshitaro Matsushita, Shunichi Sato

Author Affiliations +

Proceedings Volume 7897, Optical Interactions with Tissue and Cells XXII; 78971V (2011) https://doi.org/10.1117/12.875909
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The detailed mechanism of blast-induced traumatic brain injury (bTBI) has not been revealed yet. Thus, reliable laboratory animal models for bTBI are needed to investigate the possible diagnosis and treatment for bTBI. In this study, we used laser-induced shock wave (LISW) to induce TBI in rats and investigated the histopathological similarities to actual bTBI. After craniotomy, the rat brain was exposed to a single shot of LISW with a diameter of 3 mm at various laser fluences. At 24 h after LISW exposure, perfusion fixation was performed and the extracted brain was sectioned; the sections were stained with hematoxylin-eosin. Evans blue (EB) staining was also used to evaluate disruption of the blood brain barrier. At certain laser fluence levels, neural cell injury and hemorrhagic lesions were observed in the cortex and subcortical region. However, injury was limited in the tissue region that interacted with the LISW. The severity of injury increased with increasing laser fluence and hence peak pressure of the LISW. Fluorescence originating from EB was diffusively observed in the injuries at high fluence levels. Due to the grade and spatial controllability of injuries and the histological observations similar to those in actual bTBI, brain injuries caused by LISWs would be useful models to study bTBI.

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Ben Hatano, Yoshihisa Matsumoto, Naoki Otani, Daizoh Saitoh, Shinichi Tokuno, Yasushi Satoh, Hiroshi Nawashiro, Yoshitaro Matsushita, and Shunichi Sato "Traumatic brain injury caused by laser-induced shock wave in rats: a novel laboratory model for studying blast-induced traumatic brain injury", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78971V (18 February 2011); https://doi.org/10.1117/12.875909

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