High speed inverted optical-resolution photoacoustic microscopy

Bin Rao; Konstantin Maslov; Amos Danielli; Ruiming Chen; K. Kirk Shung; Qifa Zhou; Lihong V. Wang

doi:10.1117/12.873568

18 February 2011 High speed inverted optical-resolution photoacoustic microscopy

Bin Rao, Konstantin Maslov, Amos Danielli, Ruiming Chen, K. Kirk Shung, Qifa Zhou, Lihong V. Wang

Proceedings Volume 7899, Photons Plus Ultrasound: Imaging and Sensing 2011; 78990T (2011) https://doi.org/10.1117/12.873568
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nano-wire imaging, which is 2.0 times below the diffraction limit.

Citation Download Citation

Bin Rao, Konstantin Maslov, Amos Danielli, Ruiming Chen, K. Kirk Shung, Qifa Zhou, and Lihong V. Wang "High speed inverted optical-resolution photoacoustic microscopy", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78990T (18 February 2011); https://doi.org/10.1117/12.873568

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KEYWORDS

Imaging systems

Transducers

Ultrasonics

Pulsed laser operation

Gold

3D image processing

Image resolution

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