Visualization of heat propagation in biological tissues with two-photon fluorescence microscopy

Chih-Yuan Yang; Chien-Sheng Liao; Yu-Yi Tzeng; Shi-Wei Chu

doi:10.1117/12.875026

11 February 2011 Visualization of heat propagation in biological tissues with two-photon fluorescence microscopy

Chih-Yuan Yang, Chien-Sheng Liao, Yu-Yi Tzeng, Shi-Wei Chu

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 790338 (2011) https://doi.org/10.1117/12.875026
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In this study, we have demonstrated by using time-lapsed two-photon fluorescence microscopy (2PFM), heat flow in biological tissues can be monitored based on temperature-induced fluorescence change of endogenous molecules. Compared to conventional infrared thermography or other thermal imaging tools, 2PFM provides not only sub-micrometer spatial resolution, but also optical sectioning capability inside tissues. As a proof of principle, 2PFM was used to monitor the fluorescence variation in a leaf of Eucalyptus Robusta Smith, which is placed on a home-made heater. By heating the leaf asymmetrically, significant changes of chlorophyll fluorescence were observed in the temperature range of 30°C to 60°C, leading to the visualization of heat conduction dynamics. From the analysis of temporal variation in fluorescence, 0.5°C thermal resolution was demonstrated and detailed temperature distribution can be quantized. Such a technique will be useful for the characterization of heat-related biophysical mechanisms with high spatial and temperature resolution in a thick tissue.

Citation Download Citation

Chih-Yuan Yang, Chien-Sheng Liao, Yu-Yi Tzeng, and Shi-Wei Chu "Visualization of heat propagation in biological tissues with two-photon fluorescence microscopy", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790338 (11 February 2011); https://doi.org/10.1117/12.875026

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