FRET as a tool for the investigation of the fate of Lipidots contrast agents in vivo

J. Gravier; L. Sancey; J. L. Coll; S. Hirsjärvi; J. P. Benoît; F. Vinet; I. Texier

doi:10.1117/12.873699

11 February 2011 FRET as a tool for the investigation of the fate of Lipidots contrast agents in vivo

J. Gravier, L. Sancey, J. L. Coll, S. Hirsjärvi, J. P. Benoît, F. Vinet, I. Texier

Proceedings Volume 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III; 79100W (2011) https://doi.org/10.1117/12.873699
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Different approaches have been reported in the recent years for the in vivo delivery and targeting of poorly soluble contrast agents and active ingredients in diseased tissues. In this context, we developed new lipid nanoparticles (Lipidots®) with size being easily varied from 25 to 120 nm. Lipidots® display numerous advantages: they are composed of low-cost and biocompatible lipids; they can be stored in injection-ready formulations for long duration; their manufacturing process is versatile and up-scalable. Several indocyanines have been efficiently encapsulated in the particles while retaining their spectroscopic properties, with emission wavelengths ranging from 500 to 820 nm. Thus, dye loaded-Lipidots® have been proved suitable for both in vitro and in vivo applications. To better understand Lipidots®' behavior in biological systems, formulations based on Förster Resonance Energy Transfer (FRET) have been studied. Different pairs of the selected indocyanines were co-encapsulated and calculations proved that transfer efficiency within nanoparticles (i) behaves as in a continuous medium; (ii) depends on local acceptor concentration. Thanks to the local dye concentration dependence of FRET, these formulations are used to understand where and when the particles are assimilated in biological systems. FRET-based Lipidots® contrast agents are also promising tools to enhance imaging contrast in vivo by making a clear difference between circulating and uptaken particles.

Citation Download Citation

J. Gravier, L. Sancey, J. L. Coll, S. Hirsjärvi, J. P. Benoît, F. Vinet, and I. Texier "FRET as a tool for the investigation of the fate of Lipidots contrast agents in vivo", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100W (11 February 2011); https://doi.org/10.1117/12.873699

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available