Digital holographic microscopy of optically trapped three-dimensional microstructures

Ali-Reza Moradi; Arun Anand; Mohammad Kutub Ali; Bahram Javidi

doi:10.1117/12.885192

20 May 2011 Digital holographic microscopy of optically trapped three-dimensional microstructures

Ali-Reza Moradi, Arun Anand, Mohammad Kutub Ali, Bahram Javidi

Proceedings Volume 8043, Three-Dimensional Imaging, Visualization, and Display 2011; 804310 (2011) https://doi.org/10.1117/12.885192
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Digital holographic microscopy (DHM) provides a non-destructive and quantitative phase contrast imaging that is suitable for high resolving investigations of living cells. On the other hand, for many applications including cell analysis the immobilization of the sample under study is a crucial task. Optical trap is an elegant candidate to immobilize the micro samples. In this work, DHM is integrated with an optical trapping setup. This combination is of particular advantage for quantitative visualization of three dimensional structures that are trapped by laser beam. The recorded hologram by CCD can be post-processed to three dimensional (3D) reconstruction of the trapped objects.

Citation Download Citation

Ali-Reza Moradi, Arun Anand, Mohammad Kutub Ali, and Bahram Javidi "Digital holographic microscopy of optically trapped three-dimensional microstructures", Proc. SPIE 8043, Three-Dimensional Imaging, Visualization, and Display 2011, 804310 (20 May 2011); https://doi.org/10.1117/12.885192

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