In incubator live cell imaging platform

Nicola Moscelli; Wojciech Witarski; Sander van den Driesche; Michael J. Vellekoop

doi:10.1117/12.886908

5 May 2011 In incubator live cell imaging platform

Nicola Moscelli, Wojciech Witarski, Sander van den Driesche, Michael J. Vellekoop

Proceedings Volume 8066, Smart Sensors, Actuators, and MEMS V; 80661M (2011) https://doi.org/10.1117/12.886908
Event: SPIE Microtechnologies, 2011, Prague, Czech Republic

Abstract

In this contribution we present an imaging platform that operates in standard incubators, allowing the investigation of multiple cell cultures at the same time. Also, the sensor platform operates with disposable multi-well plates. The system consists of four image sensors (Charge-Coupled Devices) built into a custom made holder, in which a multi-well plate can be positioned without the need of further alignment. The focal points of the image sensors are fixed at the bottom of the wells of interest. Above the multi-well plate, white light emitting LEDs with an aperture are mounted to provide orthogonal illumination over the sensors. For the observation and understanding of tumour progression, cell proliferation and cell motility studies are of great importance. For such studies, a sensor system that monitors cell motility in four wells simultaneously (of a 24-well plate) has been designed and realized. In the present work we focus on measuring the motility of adherently grown mammalian epithelial cells. Simultaneous, real-time observation of stimulated (with hepatocyte growth factor) and control samples of MDCK (Madin-Darby Canine Kidney) cells has been carried out. The presented imaging platform is an attractive and versatile alternative to conventional time-lapse microscopy to monitor the motility of individual cells. Furthermore, the high-throughput feature makes its use advantageous for the simultaneous tracking of biological samples under different conditions.

Citation Download Citation

Nicola Moscelli, Wojciech Witarski, Sander van den Driesche, and Michael J. Vellekoop "In incubator live cell imaging platform", Proc. SPIE 8066, Smart Sensors, Actuators, and MEMS V, 80661M (5 May 2011); https://doi.org/10.1117/12.886908

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KEYWORDS

Sensors

Image sensors

Light emitting diodes

Live cell imaging

Carbon dioxide

CCD image sensors

Charge-coupled devices

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