Carbon nanotube-mediated siRNA delivery for gene silencing in cancer cells

Tu Hong; Honglian Guo; Yaqiong Xu

doi:10.1117/12.894162

12 September 2011 Carbon nanotube-mediated siRNA delivery for gene silencing in cancer cells

Tu Hong, Honglian Guo, Yaqiong Xu

Proceedings Volume 8099, Biosensing and Nanomedicine IV; 80990H (2011) https://doi.org/10.1117/12.894162
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

Small interfering RNA (siRNA) is potentially a promising tool in influencing gene expression with a high degree of target specificity. However, its poor intracellular uptake, instability in vivo, and non-specific immune stimulations impeded its effect in clinical applications. In this study, carbon nanotubes (CNTs) functionalized with two types of phospholipid-polyethylene glycol (PEG) have shown capabilities to stabilize siRNA in cell culture medium during the transfection and efficiently deliver siRNA into neuroblastoma and breast cancer cells. Moreover, the intrinsic optical properties of CNTs have been investigated through absorption and fluorescence measurements. We have found that the directly-functionalized groups play an important role on the fluorescence imaging of functionalized CNTs. The unique fluorescence imaging and high delivery efficiency make CNTs a promising material to deliver drugs and evaluate the treatment effect simultaneously.

Citation Download Citation

Tu Hong, Honglian Guo, and Yaqiong Xu "Carbon nanotube-mediated siRNA delivery for gene silencing in cancer cells", Proc. SPIE 8099, Biosensing and Nanomedicine IV, 80990H (12 September 2011); https://doi.org/10.1117/12.894162

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KEYWORDS

Luminescence

Absorption

Cancer

Near infrared

Optical properties

Image processing

Imaging systems

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