Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions

Anna Bar; Nicholas Snavely; Nathaniel Chen; Steven Jacques; Daniel S. Gareau

doi:10.1117/12.909902

22 February 2012 Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions

Anna Bar, Nicholas Snavely, Nathaniel Chen, Steven Jacques, Daniel S. Gareau

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Proceedings Volume 8214, Advanced Biomedical and Clinical Diagnostic Systems X; 821416 (2012) https://doi.org/10.1117/12.909902
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Screening cancer in excision margins may be done with confocal microscopy to save time and cost over the gold standard histopathology (H&E). However, diagnostic accuracy requires sufficient contrast. Reflectance mode enables detection of large (>500um) nodular tumors. Enhanced nuclear contrast with acridine orange fluorescence mode additionally enables detection of tiny (<50um) basal cell carcinomas. Here, we present a novel combination of three modes to detect squamous cell carcinoma (SCC). Accurate screening of SCC requires eosin fluorescence, reflectance and acridine orange fluorescence to enable contrast for cytoplasm, collagen and nuclei respectively. Combining these signals replicates H&E for rapid clinical translation.

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Anna Bar, Nicholas Snavely, Nathaniel Chen, Steven Jacques, and Daniel S. Gareau "Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions", Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 821416 (22 February 2012); https://doi.org/10.1117/12.909902

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KEYWORDS

Confocal microscopy

Tissues

Tumors

Luminescence

Diagnostics

Mohs surgery

Reflectivity

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