Fluorescence-enhanced optical tomography and nuclear imaging system for small animals

I-Chih Tan; Yujie Lu; Chinmay Darne; John C. Rasmussen; Banghe Zhu; Ali Azhdarinia; Shikui Yan; Anne M. Smith; Eva M. Sevick-Muraca

doi:10.1117/12.909113

29 February 2012 Fluorescence-enhanced optical tomography and nuclear imaging system for small animals

I-Chih Tan, Yujie Lu, Chinmay Darne, John C. Rasmussen, Banghe Zhu, Ali Azhdarinia, Shikui Yan, Anne M. Smith, Eva M. Sevick-Muraca

Author Affiliations +

Proceedings Volume 8216, Multimodal Biomedical Imaging VII; 82160J (2012) https://doi.org/10.1117/12.909113
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Near-infrared (NIR) fluorescence is an alternative modality for molecular imaging that has been demonstrated in animals and recently in humans. Fluorescence-enhanced optical tomography (FEOT) using continuous wave or frequency domain photon migration techniques could be used to provide quantitative molecular imaging in vivo if it could be validated against "gold-standard," nuclear imaging modalities, using dual-labeled imaging agents. Unfortunately, developed FEOT systems are not suitable for incorporation with CT/PET/SPECT scanners because they utilize benchtop devices and require a large footprint. In this work, we developed a miniaturized fluorescence imaging system installed in the gantry of the Siemens Inveon PET/CT scanner to enable NIR transillumination measurements. The system consists of a CCD camera equipped with NIR sensitive intensifier, a diode laser controlled by a single board compact controller, a 2-axis galvanometer, and RF circuit modules for homodyne detection of the phase and amplitude of fluorescence signals. The performance of the FEOT system was tested and characterized. A mouse-shaped solid phantom of uniform optical properties with a fluorescent inclusion was scanned using CT, and NIR fluorescence images at several projections were collected. The method of high-order approximation to the radioactive transfer equation was then used to reconstruct the optical images. Dual-labeled agents were also used on a tumor bearing mouse to validate the results of the FEOT against PET/CT image. The results showed that the location of the fluorophore obtained from the FEOT matches the location of tumor obtained from the PET/CT images. Besides validation of FEOT, this hybrid system could allow multimodal molecular imaging (FEOT/PET/CT) for small animal imaging.

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I-Chih Tan, Yujie Lu, Chinmay Darne, John C. Rasmussen, Banghe Zhu, Ali Azhdarinia, Shikui Yan, Anne M. Smith, and Eva M. Sevick-Muraca "Fluorescence-enhanced optical tomography and nuclear imaging system for small animals", Proc. SPIE 8216, Multimodal Biomedical Imaging VII, 82160J (29 February 2012); https://doi.org/10.1117/12.909113

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