In vivo photoacoustic imaging of tyrosinase expressing tumours in mice

Jan Laufer; Amit Jathoul; Peter Johnson; Edward Zhang; Mark Lythgoe; R. Barbara Pedley; Martin Pule; Paul Beard

doi:10.1117/12.907006

23 February 2012 In vivo photoacoustic imaging of tyrosinase expressing tumours in mice

Jan Laufer, Amit Jathoul, Peter Johnson, Edward Zhang, Mark Lythgoe, R. Barbara Pedley, Martin Pule, Paul Beard

Proceedings Volume 8223, Photons Plus Ultrasound: Imaging and Sensing 2012; 82230M (2012) https://doi.org/10.1117/12.907006
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Two human tumour cell lines (K562, 293T) were stably transfected to achieve the genetic expression of tyrosinase, which is involved in the production of the pigment eumelanin. The cells were injected subcutaneously into nude mice to form tumour xenografts, which were imaged over a period of up to 26 days using an all-optical photoacoustic imaging system. 3D photoacoustic images of the tumours and the surrounding vasculature were acquired at excitation wavelengths ranging from 600nm to 770nm. The images showed tumour growth and continued tyrosinase expression over the full 26 day duration of the study. These findings were confirmed by histological analysis of excised tumour samples.

Citation Download Citation

Jan Laufer, Amit Jathoul, Peter Johnson, Edward Zhang, Mark Lythgoe, R. Barbara Pedley, Martin Pule, and Paul Beard "In vivo photoacoustic imaging of tyrosinase expressing tumours in mice", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82230M (23 February 2012); https://doi.org/10.1117/12.907006

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available