Mosaicing for fast wide-field-of-view optical resolution photoacoustic microscopy

Peng Shao; Wei Shi; Ryan K. Chee; Alexander Forbrich; Roger J. Zemp

doi:10.1117/12.909764

23 February 2012 Mosaicing for fast wide-field-of-view optical resolution photoacoustic microscopy

Peng Shao, Wei Shi, Ryan K. Chee, Alexander Forbrich, Roger J. Zemp

Proceedings Volume 8223, Photons Plus Ultrasound: Imaging and Sensing 2012; 822311 (2012) https://doi.org/10.1117/12.909764
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The acquisition speed of previously reported mechanically-scanned Optical-Resolution Photoacoustic Microscopy (OR-PAM) systems has been limited by both laser pulse repetition rate and mechanical scanning speed. In this paper we introduce a mosaicing scheme wherein a grid of small sub-mm-scale field-of-view (FOV) patches are acquired in 0.5s per patch, and a 3-axis stepper-motor system is used to mechanically move the object to be imaged from patch-to-patch in less than 0.5s. Patch images are aligned and stitched to generate a large FOV image composite. This system retains the SNR-advantages of focused-transducer OR-PAM systems, and is a hybrid approach between optical-scanning and mechanical scanning. With this strategy we reduce the data acquisition time of previously reported large-FOV systems by a factor of around 23. SCID hairless mice are imaged. The wide-FOV, high-speed data acquisition OR-PAM system broadens the potential applications of the imaging modality.

Citation Download Citation

Peng Shao, Wei Shi, Ryan K. Chee, Alexander Forbrich, and Roger J. Zemp "Mosaicing for fast wide-field-of-view optical resolution photoacoustic microscopy", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 822311 (23 February 2012); https://doi.org/10.1117/12.909764

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