Quantitative second-harmonic generation imaging to detect osteogenesis imperfecta in human skin samples

J. Adur; A. E. Ferreira; L. D'Souza-Li; V. B. Pelegati; A. A. de Thomaz; D. B. Almeida; M. O. Baratti; H. F. Carvalho; C. L. Cesar

doi:10.1117/12.907320

13 February 2012 Quantitative second-harmonic generation imaging to detect osteogenesis imperfecta in human skin samples

J. Adur, A. E. Ferreira, L. D'Souza-Li, V. B. Pelegati, A. A. de Thomaz, D. B. Almeida, M. O. Baratti, H. F. Carvalho, C. L. Cesar

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82263P (2012) https://doi.org/10.1117/12.907320
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Osteogenesis Imperfecta (OI) is a genetic disorder that leads to bone fractures due to mutations in the Col1A1 or Col1A2 genes that affect the primary structure of the collagen I chain with the ultimate outcome in collagen I fibrils that are either reduced in quantity or abnormally organized in the whole body. A quick test screening of the patients would largely reduce the sample number to be studied by the time consuming molecular genetics techniques. For this reason an assessment of the human skin collagen structure by Second Harmonic Generation (SHG) can be used as a screening technique to speed up the correlation of genetics/phenotype/OI types understanding. In the present work we have used quantitative second harmonic generation (SHG) imaging microscopy to investigate the collagen matrix organization of the OI human skin samples comparing with normal control patients. By comparing fibril collagen distribution and spatial organization, we calculated the anisotropy and texture patterns of this structural protein. The analysis of the anisotropy was performed by means of the two-dimensional Discrete Fourier Transform and image pattern analysis with Gray-Level Co-occurrence Matrix (GLCM). From these results, we show that statistically different results are obtained for the normal and disease states of OI.

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J. Adur, A. E. Ferreira, L. D'Souza-Li, V. B. Pelegati, A. A. de Thomaz, D. B. Almeida, M. O. Baratti, H. F. Carvalho, and C. L. Cesar "Quantitative second-harmonic generation imaging to detect osteogenesis imperfecta in human skin samples", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263P (13 February 2012); https://doi.org/10.1117/12.907320

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KEYWORDS

Collagen

Second-harmonic generation

Skin

Tissues

Bone

Statistical analysis

Image analysis

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