Multiphoton imaging with compact semiconductor disk lasers

Pablo Loza-Alvarez; Rodrigo Aviles-Espinosa; David Artigas-García; Craig Hamilton; Graeme Malcolm

doi:10.1117/12.907456

14 February 2012 Multiphoton imaging with compact semiconductor disk lasers

Pablo Loza-Alvarez, Rodrigo Aviles-Espinosa, David Artigas-García, Craig Hamilton, Graeme Malcolm

Proceedings Volume 8242, Vertical External Cavity Surface Emitting Lasers (VECSELs) II; 82420O (2012) https://doi.org/10.1117/12.907456
Event: SPIE LASE, 2012, San Francisco, California, United States

Abstract

We present the performance of a compact, non expensive, easy to use ultrafast semiconductor disk laser (1W average power, 1.5 ps, 500MHz) for multiphoton imaging. The laser's operating wavelengths of 970 nm makes it ideal for nonlinear excitation of GFP as it has a two-photon action cross section peak at this wavelength. This property relaxes the required peak powers for TPEF imaging. We show the suitability of this laser for in-vivo imaging with GFP and other dyes; at different penetration depths; time-lapse studies and SHG imaging. The laser performance is evaluated in commercial microscopes and in comparison with Ti:sapphire lasers.

Citation Download Citation

Pablo Loza-Alvarez, Rodrigo Aviles-Espinosa, David Artigas-García, Craig Hamilton, and Graeme Malcolm "Multiphoton imaging with compact semiconductor disk lasers", Proc. SPIE 8242, Vertical External Cavity Surface Emitting Lasers (VECSELs) II, 82420O (14 February 2012); https://doi.org/10.1117/12.907456

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