Line-scan focal modulation microscopy for rapid imaging of thick biological specimens

Shau Poh Chong; Shilpa Pant; Nanguang Chen

doi:10.1117/12.904115

29 November 2011 Line-scan focal modulation microscopy for rapid imaging of thick biological specimens

Shau Poh Chong, Shilpa Pant, Nanguang Chen

Proceedings Volume 8311, Optical Sensors and Biophotonics III; 83111C (2011) https://doi.org/10.1117/12.904115
Event: SPIE/OSA/IEEE Asia Communications and Photonics, 2011, Shanghai, China

Abstract

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal Modulation Microscopy [1-3] (FMM) is an emerging single-photon excitation fluorescence microscopy technique that can provide sub-micron spatial resolution for deep imaging of biological tissues mainly by preserving the signal-to-background ratio. Here we report a line-scan FMM that enables line-byline recording [4] at video frame rates (>30 fps) depending on the size of region of interest.

Citation Download Citation

Shau Poh Chong, Shilpa Pant, and Nanguang Chen "Line-scan focal modulation microscopy for rapid imaging of thick biological specimens", Proc. SPIE 8311, Optical Sensors and Biophotonics III, 83111C (29 November 2011); https://doi.org/10.1117/12.904115

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