Ultrasensitive electrochemiluminescence detection of thrombin based on aptamer and cystamine modified gold nanoparticle probe

Ruixue Duan; Xiaoming Zhou

doi:10.1117/12.918830

13 March 2012 Ultrasensitive electrochemiluminescence detection of thrombin based on aptamer and cystamine modified gold nanoparticle probe

Ruixue Duan, Xiaoming Zhou

Author Affiliations +

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290D (2012) https://doi.org/10.1117/12.918830
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Recently, our group showed that one can detect specific oligonucleotides at low femtomolar levels with the electrochemiluminescence (ECL) biobarcode approach based on tris-(2, 2'-bipyridyl) ruthenium (TBR)-labeled cysteamine. It would be a significant advance to use the cysteamine assisted ECL biobarcode assay to detect protein targets in addition to DNA targets. Taking advantage of sandwich binding of two affinity aptamers for increased specificity, TBR-cysteamine as biobarcode for signal amplification and magnetic beads based ECL technology for rapid detection, a promising assay for thrombin quantification is developed. The sandwich complex could be selectively captured by micromagnetic particles and then quantified by ECL signals. Current cysteamine-Gold nanoparticle (GNP) conjugates based ECL biobarcode assay is expected to become a powerful tool for protein analysis.

Citation Download Citation

Ruixue Duan and Xiaoming Zhou "Ultrasensitive electrochemiluminescence detection of thrombin based on aptamer and cystamine modified gold nanoparticle probe", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290D (13 March 2012); https://doi.org/10.1117/12.918830

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available