Application of x-ray nano-particulate markers for the visualization of intermediate layers and interfaces using scanning electron microscopy

Nadezda O. Bessudnova; David I. Bilenko; Andrey M. Zakharevich

doi:10.1117/12.924145

28 February 2012 Application of x-ray nano-particulate markers for the visualization of intermediate layers and interfaces using scanning electron microscopy

Nadezda O. Bessudnova, David I. Bilenko, Andrey M. Zakharevich

Author Affiliations +

Proceedings Volume 8337, Saratov Fall Meeting 2011: Optical Technologies in Biophysics and Medicine XIII; 833709 (2012) https://doi.org/10.1117/12.924145
Event: Saratov Fall Meeting 2011, 2011, Saratov, Russian Federation

Abstract

In this study the methodology of biological sample preparation for dental research using SEM/EDX has been elaborated. (1)The original cutting equipment supplied with 3D user-controlled sample fixation and an adjustable cooling system has been designed and evaluated. (2) A new approach to the root dentine drying procedure has been developed to preserve structure peculiarities of root dentine. (3) A novel adhesive system with embedded X-Ray nanoparticulate markers has been designed. (4)The technique allowing for visualization of bonding resins, interfaces and intermediate layers between tooth hard tissues and restorative materials of endodontically treated teeth using the X-ray nano-particulate markers has been developed and approved. These methods and approaches were used to compare the objective depth of penetration of adhesive systems of different generations in root dentine. It has been shown that the depth of penetration in dentine is less for adhesive systems of generation VI in comparison with bonding resins of generation V, which is in agreement with theoretical evidence. The depth of penetration depends on the correlation between the direction of dentinal tubules, bonding resin delivery and gravity.

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Nadezda O. Bessudnova, David I. Bilenko, and Andrey M. Zakharevich "Application of x-ray nano-particulate markers for the visualization of intermediate layers and interfaces using scanning electron microscopy", Proc. SPIE 8337, Saratov Fall Meeting 2011: Optical Technologies in Biophysics and Medicine XIII, 833709 (28 February 2012); https://doi.org/10.1117/12.924145

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