Self-interference digital holographic microscopy for live cell imaging

Björn Kemper; Sebastian Dartmann; Frank Schlichthaber; Angelika Vollmer; Steffi Ketelhut; Gert von Bally

doi:10.1117/12.921857

8 May 2012 Self-interference digital holographic microscopy for live cell imaging

Björn Kemper, Sebastian Dartmann, Frank Schlichthaber, Angelika Vollmer, Steffi Ketelhut, Gert von Bally

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 842709 (2012) https://doi.org/10.1117/12.921857
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

Quantitative digital holographic multi-focus phase imaging enables label-free minimally invasive live cell analysis by high resolution detection of sample induced optical path length changes. However, a drawback of many experimental arrangements for the analysis of living cells with digital holography is the requirement for a separate reference wave which results in a phase stability decrease and the demand for a precise adjustment of the intensity ratio between object and reference wave. Thus, a self interference digital holographic microscopy (DHM) approach was explored which only requires a single object illumination wave. Due to the Michelson interferometer design of the proposed experimental setup two wave fronts with an almost identical curvature are superimposed. This results in a simplified evaluation of the digital holograms. The applicability of the proposed self interference principle is illustrated by results from a technical specimen and living single cells. Furthermore, adherent cancer cells have been analyzed for morphology changes in perfusion chambers due to flow and the refractive index of suspended cells was determined. In summary, the method prospects to be a versatile tool for quantitative phase imaging as simplification is important for the establishment of these methods in live cell analysis.

Citation Download Citation

Björn Kemper, Sebastian Dartmann, Frank Schlichthaber, Angelika Vollmer, Steffi Ketelhut, and Gert von Bally "Self-interference digital holographic microscopy for live cell imaging", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842709 (8 May 2012); https://doi.org/10.1117/12.921857

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available