The development of skin immersion clearing method for increasing of laser exposure efficiency on subcutaneous objects

Alexandra M. Kozina; Elina A. Genina; Georgy S. Terentyuk; Artem G. Terentyuk; Alexey N. Bashkatov; Valery V. Tuchin; Boris N. Khlebtsov

doi:10.1117/12.922725

8 May 2012 The development of skin immersion clearing method for increasing of laser exposure efficiency on subcutaneous objects

Alexandra M. Kozina, Elina A. Genina, Georgy S. Terentyuk, Artem G. Terentyuk, Alexey N. Bashkatov, Valery V. Tuchin, Boris N. Khlebtsov

Author Affiliations +

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 842726 (2012) https://doi.org/10.1117/12.922725
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

In this paper we have studied effect of a hyperosmotic optical clearing agent (OCA), such as polyethylene glycol, on the fluorescence intensity from a target located in subcutaneous area in the model experiments. As a fluorescence agent the nanocomposite including gold nanorods with hematophorphyrin was used. The remitted fluorescent signal traveling to the tissue surface was monitored over time as the tissue was treated with the OCA. The detected fluorescent signal increased as the scattering in tissue samples was substantially reduced. The study has shown how OCA can be used to improve the detected signal at localization of subcutaneous target tissue at the photothermal or photodynamic therapy. Immersion clearing of skin can be also useful for improvement of laser exposure efficiency due to the increasing of light penetration depth.

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Alexandra M. Kozina, Elina A. Genina, Georgy S. Terentyuk, Artem G. Terentyuk, Alexey N. Bashkatov, Valery V. Tuchin, and Boris N. Khlebtsov "The development of skin immersion clearing method for increasing of laser exposure efficiency on subcutaneous objects", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842726 (8 May 2012); https://doi.org/10.1117/12.922725

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