Off-axis multispectral digital holographic microscope with partially coherent illumination

Frank Dubois; Catherine Yourassowsky

doi:10.1117/12.922005

4 May 2012 Off-axis multispectral digital holographic microscope with partially coherent illumination

Frank Dubois, Catherine Yourassowsky

Proceedings Volume 8429, Optical Modelling and Design II; 84291E (2012) https://doi.org/10.1117/12.922005
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

We developed a new off-axis digital holographic microscope (DHM), working in transmission with a RGB LED illumination. This partially coherent multi-wavenlenght source gives a low noise holograms and the intensity image quality is fully exploitable for microscopy purposes. We implemented a full off-axis configuration enables the recording of the holographic data in one shot. As we used for the first tests a monochromatic CCD camera, the holograms are recorded separately in each spectral channel. The holograms are individually processed by the Fourier method to obtain, in each color, the complex amplitudes and the corresponding intensities and optical phases. The color intensities are recombined to obtain a composite RGB color image. The digital holographic refocusing and the optical phase map computation are successfully demonstrated. The multi-wavelengths operation provides a significant improvement of the collected information for colored samples. The full one-shot color hologram recording should be available with a color camera.

Citation Download Citation

Frank Dubois and Catherine Yourassowsky "Off-axis multispectral digital holographic microscope with partially coherent illumination", Proc. SPIE 8429, Optical Modelling and Design II, 84291E (4 May 2012); https://doi.org/10.1117/12.922005

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