Metabolic changes of cultured DRG neurons induced by adenosine using confocal microscopy imaging

Liqin Zheng; Yimei Huang; Jiangxu Chen; Yuhua Wang; Hongqin Yang; Yanding Zhang; Shusen Xie

doi:10.1117/12.1000079

11 December 2012 Metabolic changes of cultured DRG neurons induced by adenosine using confocal microscopy imaging

Liqin Zheng, Yimei Huang, Jiangxu Chen, Yuhua Wang, Hongqin Yang, Yanding Zhang, Shusen Xie

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855314 (2012) https://doi.org/10.1117/12.1000079
Event: Photonics Asia, 2012, Beijing, China

Abstract

Adenosine exerts multiple effects on pain transmission in the peripheral nervous system. This study was performed to use confocal microscopy to evaluate whether adenosine could affect dorsal root ganglia (DRG) neurons in vitro and test which adenosine receptor mediates the effect of adenosine on DRG neurons. After adding adenosine with different concentration, we compared the metabolic changes by the real time imaging of calcium and mitochondria membrane potential using confocal microscopy. The results showed that the effect of 500 μM adenosine on the metabolic changes of DRG neurons was more significant than others. Furthermore, four different adenosine receptor antagonists were used to study which receptor mediated the influences of adenosine on the cultured DRG neurons. All adenosine receptor antagonists especially A₁ receptor antagonist (DPCPX) had effect on the Ca²⁺ and mitochondria membrane potential dynamics of DRG neurons. The above studies demonstrated that the effect of adenosine which may be involved in the signal transmission on the sensory neurons was dose-dependent, and all the four adenosine receptors especially the A1R may mediate the transmission.

Citation Download Citation

Liqin Zheng, Yimei Huang, Jiangxu Chen, Yuhua Wang, Hongqin Yang, Yanding Zhang, and Shusen Xie "Metabolic changes of cultured DRG neurons induced by adenosine using confocal microscopy imaging", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855314 (11 December 2012); https://doi.org/10.1117/12.1000079

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