Rabbit electroretinograms evoked by 632.8nm laser flash stimuli

Zai-Fu Yang; Hong-Xia Chen; Jia-Rui Wang; Bo-Lin Guan; Guang-Yuan Yu; Xiao-Na Zhang; Wen-Yuan Zhang; Jing-Geng Yang

doi:10.1117/12.999975

11 December 2012 Rabbit electroretinograms evoked by 632.8nm laser flash stimuli

Zai-Fu Yang, Hong-Xia Chen, Jia-Rui Wang, Bo-Lin Guan, Guang-Yuan Yu, Xiao-Na Zhang, Wen-Yuan Zhang, Jing-Geng Yang

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 85531X (2012) https://doi.org/10.1117/12.999975
Event: Photonics Asia, 2012, Beijing, China

Abstract

The flash electroretinography is a standard electrophysiological method and widely employed in basic research and ophthalmology clinics, of which the stimulus is usually white flash from dome stimulator. However, little is known about the electroretinograms (ERGs) evoked by monochromatic laser flash stimuli. The goal of this research effort is to quantify the ERGs of dark-adapted New Zealand rabbits elicited by He-Ne laser flash with wavelength 632.8 nm. The flash field was a Maxwellian viewing disc with angular subtense of 8.5°, 13.3° or 20.2°. The stimulus duration was 12 ms, 22 ms, 70 ms or 220 ms. The laser flash power incident on the cornea varied from 2.2 nW through 22 mW. Under the condition of 20 ms stimulus duration and 20.2° flash field, the ERG of New Zealand rabbit was compared with that of Chinchilla gray rabbit. Results showed that for the ERG b-wave, with the increase of laser energy, the amplitude first increased, then met a trough and finally increased again, the implicit time decreased first and then met a platform. While for the ERG a-wave, the amplitude increased and the implicit time decreased monotonically. Longer stimulus duration led to lower b-wave amplitude under equal flash power level. The flash field size showed limited effect on the ERG, especially on the low energy end. As compared with the pigmented rabbit, the albino rabbit was more sensitive and the threshold energy for b-wave excitation was about 10 times lower.

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Zai-Fu Yang, Hong-Xia Chen, Jia-Rui Wang, Bo-Lin Guan, Guang-Yuan Yu, Xiao-Na Zhang, Wen-Yuan Zhang, and Jing-Geng Yang "Rabbit electroretinograms evoked by 632.8nm laser flash stimuli", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531X (11 December 2012); https://doi.org/10.1117/12.999975

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KEYWORDS

Helium neon lasers

Laser energy

Electrodes

Retina

Amplifiers

Light emitting diodes

Cornea

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