Imaging the morphological change of tissue structure during the early phase of esophageal tumor progression using multiphoton microscopy

Jian Xu; Deyong Kang; Meifang Xu; Xiaoqin Zhu; Shuangmu Zhuo; Jianxin Chen

doi:10.1117/12.999848

11 December 2012 Imaging the morphological change of tissue structure during the early phase of esophageal tumor progression using multiphoton microscopy

Jian Xu, Deyong Kang, Meifang Xu, Xiaoqin Zhu, Shuangmu Zhuo, Jianxin Chen

Author Affiliations +

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855334 (2012) https://doi.org/10.1117/12.999848
Event: Photonics Asia, 2012, Beijing, China

Abstract

Esophageal cancer is a common malignancy with a very poor prognosis. Successful strategies for primary prevention and early detection are critically needed to control this disease. Multiphoton microscopy (MPM) is becoming a novel optical tool of choice for imaging tissue architecture and cellular morphology by two-photon excited fluorescence. In this study, we used MPM to image microstructure of human normal esophagus, carcinoma in situ (CIS), and early invasive carcinoma in order to establish the morphological features to differentiate these tissues. The diagnostic features such as the appearance of cancerous cells, the significant loss of stroma, the absence of the basement membrane were extracted to distinguish between normal and cancerous esophagus tissue. These results correlated well with the paired histological findings. With the advancement of clinically miniaturized MPM and the multi-photon probe, combining MPM with standard endoscopy will therefore allow us to make a real-time in vivo diagnosis of early esophageal cancer at the cellular level.

Citation Download Citation

Jian Xu, Deyong Kang, Meifang Xu, Xiaoqin Zhu, Shuangmu Zhuo, and Jianxin Chen "Imaging the morphological change of tissue structure during the early phase of esophageal tumor progression using multiphoton microscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855334 (11 December 2012); https://doi.org/10.1117/12.999848

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