Multiphoton gonioscopy to image the trabecular meshwork of porcine eyes

Omid Masihzadeh; David A. Ammar; Malik Y. Kahook; Emily A. Gibson; Tim C. Lei

doi:10.1117/12.2003031

26 March 2013 Multiphoton gonioscopy to image the trabecular meshwork of porcine eyes

Omid Masihzadeh, David A. Ammar, Malik Y. Kahook, Emily A. Gibson, Tim C. Lei

Proceedings Volume 8567, Ophthalmic Technologies XXIII; 85671E (2013) https://doi.org/10.1117/12.2003031
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The aqueous outflow system (AOS), including the trabecular meshwork (TM), the collector channels (CC) and the Schlemm’s canal (SC), regulates intraocular pressure (IOP) through the drainage of the aqueous humor (AH). Abnormal IOP elevation leads to increased pressure stress to retinal ganglion cells, resulting in cell loss that can ultimately lead to complete loss of eyesight. Therefore, development of imaging tools to detect abnormal structural and functional changes of the AOS is important in early diagnosis and prevention of glaucoma. Multiphoton microscopy (MPM), including twophoton autofluorescence (TPAF) and second harmonic generation (SHG), is a label-free microscopic technique that allows molecular specific imaging of biological tissues like the TM. Since the TM and other AOS structures are located behind the highly scattering scleral tissue, transscleral imaging of the TM does not provide enough optical resolution. In this work, a gonioscopic lens is used to allow direct optical access of the TM through the cornea for MPM imaging. Compared to transscleral imaging, the acquired MPM images show improved resolution as individual collagen fiber bundles of the TM can be observed. MPM gonioscopy may have the potential to be developed as a future clinical imaging tool for glaucoma diagnostics.

Citation Download Citation

Omid Masihzadeh, David A. Ammar, Malik Y. Kahook, Emily A. Gibson, and Tim C. Lei "Multiphoton gonioscopy to image the trabecular meshwork of porcine eyes", Proc. SPIE 8567, Ophthalmic Technologies XXIII, 85671E (26 March 2013); https://doi.org/10.1117/12.2003031

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