Towards microscopic resolution in holoscopy

Gesa Lilith Franke; Dierck Hillmann; Christian Lührs; Peter Koch; Jörn Wollenzin; Gereon Hüttmann

doi:10.1117/12.2006806

20 March 2013 Towards microscopic resolution in holoscopy

Gesa Lilith Franke, Dierck Hillmann, Christian Lührs, Peter Koch, Jörn Wollenzin, Gereon Hüttmann

Proceedings Volume 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII; 85711O (2013) https://doi.org/10.1117/12.2006806
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.

Citation Download Citation

Gesa Lilith Franke, Dierck Hillmann, Christian Lührs, Peter Koch, Jörn Wollenzin, and Gereon Hüttmann "Towards microscopic resolution in holoscopy", Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 85711O (20 March 2013); https://doi.org/10.1117/12.2006806

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